| 1. | These are inserted into a cloning vector .
它们被插入克隆载体。 |
| 2. | An outline of the procedure for introducing a piece of foreign dna into a cloning vector is shown in figure 1. 3 .
图13列出了引出一段外源DNA进入克隆载体的大概步骤。 |
| 3. | An outline of the procedure for introducing a piece of foreign dna into a cloning vector is shown in figure 1 . 3
图1 3列出了引出一段外源dna进入克隆载体的大概步骤。 |
| 4. | In genetic engneering , nonviral dna can be inserted into a phage , which is then used as a cloning vector
在基因工程中，没有病毒的dna可以被插入到噬菌体中，用作克隆载体。 |
| 5. | The pcr product was inserted in e . coli clone vector pgem t - easy , and sequenced . a new subtype poifn - a was obtained
Pcr产物经纯化回收后，插入pgemt - easyvector ，经序列测定证实克隆了一新亚型猪干扰素。 |
| 6. | Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants , it has been used as a cloning vector in gnetic engineering
在许多双子叶植物中质粒在寄主细胞中是独立的复制单元，所以可以在基因工程中用作克隆载体。 |
| 7. | Firstly , the hyaluronate synthase ( has ) gene ( hasa ) was cloned into the cloning vector puc19 by pcr using the total dna sample of s . equi as template
本文研究代谢工程理论，对本实验室的一株ha生产菌( streptococcusequi )进行了ha合成与分解关键酶基因及功能的研究。 |
| 8. | In our study we have cloned the osd gene from s . typhimurium by pcr , characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd
再将hpylori尿素酶b亚单位基因与尿素酶b和热休克蛋白a融合基因分别克隆入ptrc99a一asd质粒的多克隆位点之内。 |
| 9. | Recombinant cloning vector of pgem - hc and pgem - ha were constructed by ligating the hc and ha genes into pgem t east vector and transformating into jm109 . 3
分别将目的基因hypoderminc和hypodermina与克隆载体( pgemteasy )连接并转化到宿主菌jm109中，构建了重组克隆载体pgem - hc和pgem - ha 。 |
| 10. | In this experiment , ri gene was amplified by pcr method from recombinant cloning vector pt7 - ri , which had been constructed by dr . yu xiu - ping in this laboratory and sequenced by takara biotechnology ( dalian ) co . , ltd .
于秀平博士已经构建了ri的克隆载体pt7 - ri ，测序表明与已知人胎盘ri基因的cdna序列完全相同。本文的工作是在此基础之上完成的。 |
